Angiopoietin 1 Protein (ANGPT1) (AA 284-498) (DYKDDDDK Tag)
Quick Overview for Angiopoietin 1 Protein (ANGPT1) (AA 284-498) (DYKDDDDK Tag) (ABIN5564294)
Target
See all Angiopoietin 1 (ANGPT1) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 284-498
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Purification tag / Conjugate
- This Angiopoietin 1 protein is labelled with DYKDDDDK Tag.
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Purpose
- COMP (rat):Angiopoietin-1 (human) (rec.)
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Specificity
- Binds to human angiopoietin-1 receptor (Tie-2).
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Cross-Reactivity
- Human
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Characteristics
- The fibrinogen-like domain (aa 284-498) of human angiopoietin-1 is fused at the N-terminus to the coiled-coil domain of rat COMP (cartilage oligomeric matrix protein) and to a FLAG®-tag.
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Endotoxin Level
- <0.05EU/μg purified protein (LAL test).
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Biological Activity Comment
- More potent than native angiopoietin-1. The protein forms pentameric structures.
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Angiopoietin 1 (ANGPT1) (AA 20-498) protein
ANGPT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
Angiopoietin 1 (ANGPT1) protein (Fc Tag)ANGPT1 Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Angiopoietin 1 (ANGPT1) (AA 1-498) protein (GST tag)ANGPT1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Concentration
- Lot specific
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Buffer
- Contains PBS + 0.02 % CHAPS.
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Handling Advice
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.
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Storage
- 4 °C,-20 °C
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Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
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Expiry Date
- 6 months
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- Angiopoietin 1 (ANGPT1)
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Alternative Name
- Angiopoietin-1
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Background
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COMP-Ang1, COMP:Ang-1, COMP Angiopoietin 1, COMP ANGPT1
Soluble, stable and more potent angiopoietin-1 (Ang-1) variant.
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Molecular Weight
- 35kDa (SDS-PAGE)
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Pathways
- RTK Signaling, Glycosaminoglycan Metabolic Process
Target
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