FGF23 Protein (AA 1-251) (His tag)

Catalog No. ABIN5564312

Recombinant FGF23 protein expressed in HEK-293 Cells.

Quick Overview for FGF23 Protein (AA 1-251) (His tag) (ABIN5564312)

Target: FGF23 (Fibroblast Growth Factor 23 (FGF23))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: SDS-PAGE (SDS)

Purity: >90 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 1-251

Purification tag / Conjugate: This FGF23 protein is labelled with His tag.

Purpose: FGF-23 (human) (rec.) (His)

Cross-Reactivity: Human

Characteristics: Signal peptide and human FGF-23 (aa 1-251) are fused at the C-terminus to a His-tag.

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Activates ERK and FRS2alpha phosphorylation in Klotho expressing cells.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: FGF23 (Fibroblast Growth Factor 23 (FGF23))

Alternative Name: FGF-23

Background:

Fibroblast Growth Factor 23, Tumor-derived Hypophosphatemia-inducing Factor

FGF-23 (Fibroblast growth factor 23) is a regulator of phosphate homeostasis. It upregulates EGR1 expression in the presence of KLBy. Acts directly on the parathyroid to decrease PTH secretion. Regulates the vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. Defects in FGF-23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR) and of hyperphosphatemic familial tumoral calcinosis (HFTC).

Molecular Weight: ~30kDa (SDS-PAGE, full-length). FGF23 is cleaved between Arg179 and Ser180 (two bands of ~14kDa and ~16kDa).

UniProt: Q9GZV9

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Negative Regulation of Hormone Secretion