Granulin Protein (GRN) (AA 1-593)

Catalog No. ABIN5564456

Recombinant Granulin protein expressed in HEK-293 Cells.

Quick Overview for Granulin Protein (GRN) (AA 1-593) (ABIN5564456)

Target: Granulin (GRN)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: SDS-PAGE (SDS)

Purity: >98 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 1-593

Purpose: Progranulin (human) (rec.) (untagged)

Cross-Reactivity: Human

Characteristics: Signal peptide and human progranulin (aa 1-593) is untagged. Reflects the native sequence with no additional aa.

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Activates phospho-ERK1/2 in neuronal mouse P19 cells. Regulates food intake and body weight (see reference 1).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: Granulin (GRN)

Alternative Name: Progranulin

Background:

Proepithelin, PEPI, PC Cell-derived Growth Factor

Progranulin (PGRN) is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of frontotemporal degeneration (FTLD). In addition, PGRN has also been linked to tumorigenesis. Progranulin is a biomarker for FTLD, other types of Alzheimer's Disease (AD) and potentially for MCI (Mild Cognitive Impairment). Additionally, PGRN is described as a new ligand of TNF receptors and a potential therapeutic against inflammatory disease like arthritis. EphA2, a member of the large family of Ephrin receptor tyrosine kinases, is also a functional signaling receptor for progranulin as reported recently.

Molecular Weight: ~74kDa (glycosylated protein by SDS-PAGE)

UniProt: P28799