LBP Protein (His tag)

Catalog No. ABIN5663348

This Recombinant LBP protein is expressed in CHO Cells.

Quick Overview for LBP Protein (His tag) (ABIN5663348)

Target: LBP (Lipopolysaccharide Binding Protein (LBP))

Protein Type: Recombinant

Origin: Mouse

Source: CHO Cells

Application: ELISA, Agonist (Agon), Flow Cytometry (FACS)

Purity: >95 % by SDS-PAGE

Product Details

Purification tag / Conjugate: This LBP protein is labelled with His tag.

Sequence: MKAGTGPLLS TLLGLLFLSI QGTGGVNPGV VARITDKGLA YAAKEGLVAL QRELYKITLP DFSGDFKIKA VGRGQYEFHS LEIQNCELRG SSLKLLPGQG LSLAISDSSI GVRGKWKVRK SFLKLHGSFD LDVKGVTISV DLLLGMDPSG RPTVSASGCS SRICDLDVHI SGNVGWLLNL FHNQIESKLQ KVLENKVCEM IQKSVTSDLQ PYLQTLPVTA EIDNVLGIDY SLVAAPQAKA QVLDVMFKGE IFNRNHRSPV FSITDDMLPH DSGIRLNTKA FRPFTPQIYK KYPDMKLELL GTVVSAPILN ATPTPTMSLP EDSKQMVYFA ISDYAFNIAS RVYHQAGYLN VSPGNLSLAP QMEIEGFVIL PTSAREPVFR LGVVTNVFAS LTFNNSKVTG MLHPDKAQVR LIESKVGMFN VNLFQAFLNY YLLNSLYPDV NAELAQGFPL PLPRHIQLHD LDFQIRKDFL YLGANVQYMR VHHHHHH

Characteristics: Attention: His-tag at the c-terminal end of the LBP has no protease site and is not to split off

Purification: Affinity chromatography by His-Tag

Endotoxin Level: <1 EU/μg of recombinant mouse LBP as determined by LAL method

Biological Activity Comment: up to 2 μg/ ml LBP mediates binding of FITC-LPS (0.5μg/ml) to CD14+CHO transfectants (2 x 106/ml)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 40 μL sterile distilled water to a concentration of 0.25 mg/mL

Concentration: 0.25 mg/mL

Buffer: sterile-filtered phosphate balanced salt solution, pH 7.6

Preservative: Without preservative

Storage: RT,-20 °C,-80 °C

Storage Comment:

Storage: -20°C or -70°C

Storage/Stability: The lyophilized product thought stable at room temperature up to 3 weeks, but is best stored desiccated at -70oC more than 1 year, at refrigerator about 10 months. Use reconstituted protein immediately or stored in undiluted working aliquots at -20oC. For long-term storage, we recommend add of carrier protein (10% BSA). Repeated freezing and thawing should be avoided!!!The LBP will be precipitate by rapid changes of pH or temperature. Best working temperature is ice bath

Expiry Date: 12 months

Target Details

Target: LBP (Lipopolysaccharide Binding Protein (LBP))

Alternative Name: LBP

Background: Background: Natural LBP is a 58KD glycoprotein produced in liver. It binds at lipid A of LPS with high affinity (10-9M) and reduced the cellular LPS effects at CD14+ cells (IL1ß, IL6, TNFα). It acts as opsonin for GRAM negative cells, LPS, neutrophiles and granulocytes.Recombinant LBP produced from mouse LBP transfected CHO-cells in serum free medium. For transfection, we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before transfection, the complete mouse LBP cDNA amplified by PCR and cloned into expression vector p-POL-DHFR.Background: Natural LBP is a 58KD glycoprotein produced in liver. It binds at lipid A of LPS with high affinity (10-9M) and reduced the cellular LPS effects at CD14+ cells (IL1ß, IL6, TNFα). It acts as opsonin for GRAM negative cells, LPS, neutrophiles and granulocytes. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Sequence similarities belongs to the BPI/LBP/Plunc superfamily and BPI/LBP family.

Molecular Weight: ~58kDa

Gene ID: 16803

UniProt: Q61805

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process