LBP Protein (His tag)

Catalog No. ABIN5663347

Product Details

Target: LBP (Lipopolysaccharide Binding Protein (LBP))

Protein Type: Recombinant

Origin: Human

Source: CHO Cells

Purification tag / Conjugate: This LBP protein is labelled with His tag.

Application: ELISA, Agonist (Agon)

Sequence: MGALARALPS ILLALLLTST PEALGANPGL VARITDKGLQ YAAQEGLLAL QSELLRITLP DFTGDLRIPH VGRGRYEFHS LNIHSCELLH SALRPVPGQG LSLSISDSSI RVQGRWKVRK SFFKLQGSFD VSVKGISISV NLLLGSESSG RPTVTASSCS SDIADVEVDM SGDLGWLLNL FHNQIESKFQ KVLESRICEM IQKSVSSDLQ PYLQTLPVTT EIDSFADIDY SLVEAPRATA QMLEVMFKGE IFHRNHRSPV FSPGNLSVDP YMEIDAFVLL PSSSKEPVFR LSVATNVSAT LTFNTSKITG FSITDDMIPP DSNIRLTTKS FRPFVPRLAR LYPNMNLELQ GSVPSAPLLN TLLAAVMSLP EEHNKMVYFA ISDYVFNTAS LVYHEEGYLN FLKPGKVKVE LKESKVGLFN AELLEALLNY YILNTFYPKF NDKLAEGFPL PLLKRVQLYD LGLQIHKDFL FLGANVQYMR VHHHHHH

Characteristics: Attention: His-tag at the c-terminal end of the LBP has no protease site and is not to split off

Purification: Affinity chromatography by His-Tag

Purity: >95 % by SDS-PAGE

Endotoxin Level: <1 EU/μgof recombinant human LBP as determined by LAL method

Biological Activity Comment: Don't activate cells in MNC culture.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 40 μL sterile distilled water to a concentration of 0.25 mg/mL

Concentration: 0.25 mg/mL

Buffer: sterile-filtered phosphate balanced salt solution, pH 7.4

Preservative: Without preservative

Storage: RT,-20 °C,-80 °C

Storage Comment:

Storage: -20°C or -70°C

Storage/Stability: Long time -80oC. Short time storage is possible at 40C or -20C .The lyophilized product thought stable room temperature up to 3 weeks, but is best stored desiccated at -70oC more than 1 year, at refrigerator about 10 months. Reconstituted protein should be used immediately or stored in undiluted working aliquots at -20oC. For long term storage it is recommended to add a carrier protein (o.1% BSA). Repeated freezing and thawing should be avoided!!!

Expiry Date: 12 months

Target Details

Target: LBP (Lipopolysaccharide Binding Protein (LBP))

Alternative Name: LBP (LBP Products)

Synonyms: BPIFD2 Protein, Bpifd2 Protein, Ly88 Protein, LPSBP Protein, LBP Protein, lipopolysaccharide binding protein Protein, lipopolysaccharide-binding protein Protein, LBP Protein, Lbp Protein, LOC100472839 Protein

Background: Background: Natural LBP is a 58KD glycoprotein produced in liver. It binds at lipid A of LPS with high affinity (10-9M) and reduced the cellular LPS effects at CD14+ cells (IL1ß, IL6, TNFα). It acts as opsonin for GRAM negative cells, LPS, neutrophiles and granulocytes. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Recombinant LBP produced from mouse LBP transfected CHO-cells in serum free medium. For transfection, we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before transfection, the complete mouse LBP cDNA amplified by PCR and cloned into expression vector p-POL-DHFR. Sequence similarities belongs to the BPI/LBP/Plunc superfamily and BPI/LBP family.

Molecular Weight: ~58kDa

Gene ID: 3929

NCBI Accession: NP_004130

UniProt: P18428

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process