2B4 Protein (AA 22-221) (His tag,AVI tag,Biotin)

Catalog No. ABIN5674581

Recombinant 2B4 protein expressed in HEK-293 Cells.

Quick Overview for 2B4 Protein (AA 22-221) (His tag,AVI tag,Biotin) (ABIN5674581)

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 22-221

Purification tag / Conjugate: This 2B4 protein is labelled with His tag,AVI tag,Biotin.

Brand: MABSol® - PrecisionAvi

Sequence: AA 22-221

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 25.4 kDa. The protein migrates as 40-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
This product is biotinylated with precision by using the Avitag™ technology, resulting in precise, single residue biotinylation at the lysine of the tag. This leads to minimal interence with protein binding, structure, and function, as well as uniform orientation upon immobilization.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Alternative Name: 2B4

Background: Natural killer cell receptor 2B4 is also known as NK cell type I receptor protein 2B4 (NKR2B4 or h2B4), SLAM family member 4 (SLAMF4), Signaling lymphocytic activation molecule 4, CD antigen CD244. NKR2B4 / CD244 contains two Ig-like (immunoglobulin-like) domains. CD244 is expressed in spleen, PBL, followed by lung, liver, testis and small intestine. CD244 interacts with CD48. Following phosphorylation, CD244 is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. SLAMF4 modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family.

Molecular Weight: 25.4 kDa

NCBI Accession: NP_057466