Maltose Binding Protein Protein (MBP) (AA 27-392) (His tag)

Catalog No. ABIN5853046

Recombinant Maltose Binding Protein protein expressed in Escherichia coli (E. coli).

Quick Overview for Maltose Binding Protein Protein (MBP) (AA 27-392) (His tag) (ABIN5853046)

Target: Maltose Binding Protein (MBP)

Protein Type: Recombinant

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % by SDS - PAGE

Product Details

Protein Characteristics: AA 27-392

Purification tag / Conjugate: This Maltose Binding Protein protein is labelled with His tag.

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMKIEEGK LVIWINGDKG YNGLAEVGKK FEKDTGIKVT VEHPDKLEEK FPQVAATGDG PDIIFWAHDR FGGYAQSGLL AEITPDKAFQ DKLYPFTWDA VRYNGKLIAY PIAVEALSLI YNKDLLPNPP KTWEEIPALD KELKAKGKSA LMFNLQEPYF TWPLIAADGG YAFKYENGKY DIKDVGVDNA GAKAGLTFLV DLIKNKHMNA DTDYSIAEAA FNKGETAMTI NGPWAWSNID TSKVNYGVTV LPTFKGQPSK PFVGVLSAGI NAASPNKELA KEFLENYLLT DEGLEAVNKD KPLGAVALKS YEEELAKDPR IAATMENAQK GEIMPNIPQM SAFWYAVRTA VINAASGRQT VDEALKDAQT NSSSNNNNNN NNNNLGIEGR

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.1M NaCl, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Maltose Binding Protein (MBP)

Alternative Name: maltose Binding Protein

Background: Cytoplasmic maltose-binding protein, also known as MBP, is a protein related with the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has been used to increase the yield of its fusion partner in many cases. In addition, MBP is often able to promote the solubility of polypeptides to which it is fused. Recombinant E.coli MBP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 44.9kDa (410aa), confirmed by MALDI-TOF

NCBI Accession: NP_418458

UniProt: P0AEX9