Prothrombin Protein (AA 328-622)

Catalog No. ABIN5853681

This Recombinant Prothrombin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Prothrombin Protein (AA 328-622) (ABIN5853681)

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 80 %

Product Details

Protein Characteristics: AA 328-622

Sequence: MTFGSGEADC GLRPLFEKKS LEDKTERELL ESYIDGRIVE GSDAEIGMSP WQVMLFRKSP QELLCGASLI SDRWVLTAAH CLLYPPWDKN FTENDLLVRI GKHSRTRYER NIEKISMLEK IYIHPRYNWR ENLDRDIALM KLKKPVAFSD YIHPVCLPDR ETAASLLQAG YKGRVTGWGN LKETWTANVG KGQPSVLQVV NLPIVERPVC KDSTRIRITD NMFCAGYKPD EGKRGDACEG DSGGPFVMKS PFNNRWYQMG IVSWGEGCDR DGKYGFYTHV FRLKKWIQKV IDQFGE

Purification: > 80% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Denatured

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 0.4M urea, 10 % glycerol

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Alternative Name: Coagulation Factor II/Thrombin

Background: Coagulation factor II, also known as F2, is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Recombinant human F2 protein was expressed in E. coli.

Molecular Weight: 33.9 kDa (296aa)

NCBI Accession: NP_000497

Pathways: Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling