VISTA Protein (AA 33-194) (His tag)
Quick Overview for VISTA Protein (AA 33-194) (His tag) (ABIN7676722)
Target
See all VISTA ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 33-194
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Purification tag / Conjugate
- This VISTA protein is labelled with His tag.
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Sequence
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FKVATPYSLY VCPEGQNVTL TCRLLGPVDK GHDVTFYKTW YRSSRGEVQT CSERRPIRNL TFQDLHLHHG GHQAANTSHD LAQRHGLESA SDHHGNFSIT MRNLTLLDSG LYCCLVVEIR HHHSEHRVHG AMELQVQTGK DAPSNCVVYP SSSQDSENIT AA
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Purification
- > 95% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA) protein (His tag)
VISTA Origin: Cynomolgus Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA) (AA 1-311) protein (GST tag)VISTA Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA) protein (Myc-DYKDDDDK Tag)VISTA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA) protein (DYKDDDDK-His Tag)VISTA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Phosphate-Buffered Saline ( pH 7.4)
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))
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Alternative Name
- VISTA/B7-H5/PD-1H
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Background
- C10orf54, also known as Platelet receptor Gi24, is a member of the immunoglobulin superfamily. As a transmembrane molecule, this protein is expressed in bone on embryonic stem cells (ESCs), and on tumor cell surface. Thus, C10orf54 supports the differentiation of ESC and ehhances BMP4 induced signaling in ESC, but is also down regulated following BMP4 exposure. Recombinant human C10orf54, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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Molecular Weight
- 19.1 kDa (170aa)
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NCBI Accession
- NP_071436
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Pathways
- Cancer Immune Checkpoints
Target
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