VISTA Protein (AA 33-191) (His tag)

Catalog No. ABIN7315040

This Recombinant VISTA protein is produced in Human Cells.

Quick Overview for VISTA Protein (AA 33-191) (His tag) (ABIN7315040)

Target: VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))

Protein Type: Recombinant

Origin: Mouse

Source: Human Cells

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Mass Spectrometry (MS)

Purity: Greater than 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 33-191

Purification tag / Conjugate: This VISTA protein is labelled with His tag.

Purpose: Recombinant Mouse VISTA Protein is produced by mammalian expression system and the target gene encoding Phe33-Ala191 is expressed fused with a 6His tag at the C-terminus.

Sequence: FKVTTPYSLY VCPEGQNATL TCRILGPVSK GHDVTIYKTW YLSSRGEVQM CKEHRPIRNF TLQHLQHHGS HLKANASHDQ PQKHGLELAS DHHGNFSITL RNVTPRDSGL YCCLVIELKN HHPEQRFYGS MELQVQAGKG SGSTCMASNE QDSDSITAAH HHHHH

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS,  pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))

Alternative Name: C10orf54

Pathways: Cancer Immune Checkpoints