VISTA Protein (AA 33-194) (His tag)

Catalog No. ABIN7703587

This Recombinant VISTA protein is produced in Mammalian Cells.

Quick Overview for VISTA Protein (AA 33-194) (His tag) (ABIN7703587)

Target: VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))

Protein Type: Recombinant

Origin: Human

Source: Mammalian Cells

Application: ELISA, Western Blotting (WB)

Purity: Greater than 95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 33-194

Purification tag / Conjugate: This VISTA protein is labelled with His tag.

Purpose: Recombinant Human VISTA

Endotoxin Level: < 1 EU/μg as determined by LAL test.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20°C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80°C for 3 months or 1 week at 2 to 8°C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))

Alternative Name: VISTA

Background: Platelet receptor Gi24, Stress-induced secreted protein-1, Sisp-1, C10orf54, SISP1

Molecular Weight: 30-50 kDa

Pathways: Cancer Immune Checkpoints