EPH Receptor B2 Protein (EPHB2) (AA 19-543) (His tag)

Catalog No. ABIN7281187

Recombinant EPH Receptor B2 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for EPH Receptor B2 Protein (EPHB2) (AA 19-543) (His tag) (ABIN7281187)

Target: EPH Receptor B2 (EPHB2)

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: SDS-PAGE (SDS)

Purity: > 90 % by SDS - PAGE

Product Details

Protein Characteristics: AA 19-543

Purification tag / Conjugate: This EPH Receptor B2 protein is labelled with His tag.

Sequence: VEETLMDSTT ATAELGWMVH PPSGWEEVSG YDENMNTIRT YQVCNVFESS QNNWLRTKFI RRRGAHRIHV EMKFSVRDCS SIPSVPGSCK ETFNLYYYEA DFDSATKTFP NWMENPWVKV DTIAADESFS QVDLGGRVMK INTEVRSFGP VSRSGFYLAF QDYGGCMSLI AVRVFYRKCP RIIQNGAIFQ ETLSGAESTS LVAARGSCIA NAEEVDVPIK LYCNGDGEWL VPIGRCMCKA GFEAVENGTV CRGCPSGTFK ANQGDEACTH CPINSRTTSE GATNCVCRNG YYRADLDPLD MPCTTIPSAP QAVISSVNET SLMLEWTPPR DSGGREDLVY NIICKSCGSG RGACTRCGDN VQYAPRQLGL TEPRIYISDL LAHTQYTFEI QAVNGVTDQS PFSPQFASVN ITTNQAAPSA VSIMHQVSRT VDSITLSWSQ PDQPNGVILD YELQYYEKEL SEYNATAIKS PTNTVTVQGL KAGAIYVFQV RARTVAGYGR YSGKMYFQTM TEAEYQTSIQ EKLPLLEHHH HHH

Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 20 % glycerol, 1  mM DTT.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: EPH Receptor B2 (EPHB2)

Alternative Name: EPHB2

Background: EPHB2, also known as ephrin type-B receptor 2 isoform 1, is a member of the transmembrane Eph receptor tyrosine kinase family (RTKs) that binds members of the Ephrin family on adjacent cells. The interaction triggers forward signaling in the receptor-expressing cells through the Eph receptor and reverse signaling in the ligand-expressing cells through Ephrin. Hippocampal neurons can release vesicles containing full length EPHB2, and these are taken up by neighboring glial cells. This protein controls axon guidance across the embryonic midline, promotes a neuronal fate from neural precursors, and regulates NMDA receptor activity. Recombinant human EPHB2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 59.1kDa (533aa) 50-70KDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_059145

UniProt: P29323

Pathways: RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins