Sonic Hedgehog Protein (SHH) (AA 24-197, Cys24IleIle-Mutant)
Quick Overview for Sonic Hedgehog Protein (SHH) (AA 24-197, Cys24IleIle-Mutant) (ABIN7314540)
Target
See all Sonic Hedgehog (SHH) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 24-197, Cys24IleIle-Mutant
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Purpose
- Recombinant Human SHH Protein is produced by E.coli expression system and the target gene encoding Cys24-Gly197, (Cys24Ile-Ile) is expressed.
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Sequence
- MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
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Sonic Hedgehog (SHH) (AA 25-198), (Cys25IleIle-Mutant) (Active) protein
SHH Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 24-197), (Cys24IleIle-Mutant) (Active) proteinSHH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 24-197) proteinSHH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sonic Hedgehog (SHH) protein (His tag)SHH Origin: Mouse Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (Active) proteinSHH Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) protein (His tag)SHH Origin: Human Host: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (AA 181-280) protein (GST tag)SHH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sonic Hedgehog (SHH) (Active) protein (His tag)SHH Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) (AA 25-198) protein (His tag)SHH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE SDS
Sonic Hedgehog (SHH) (AA 24-197) protein (His tag)SHH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 100 mM NaCl, 1 mM DTT, pH 7.5.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Sonic Hedgehog (SHH)
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Alternative Name
- SHH
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Gene ID
- 6469
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UniProt
- Q15465
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Pathways
- Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
Target
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