SMAD3 Protein (AA 2-425) (DYKDDDDK Tag,His tag)
Quick Overview for SMAD3 Protein (AA 2-425) (DYKDDDDK Tag,His tag) (ABIN7314542)
Target
See all SMAD3 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 2-425
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Purification tag / Conjugate
- This SMAD3 protein is labelled with DYKDDDDK Tag,His tag.
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Purpose
- Recombinant Human SMAD3 Protein is produced by E.coli expression system and the target gene encoding Ser2-Ser425 is expressed with a 6His, Flag tag at the N-terminus.
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Sequence
- MRGSHHHHHH GSDYKDDDDK SSILPFTPPI VKRLLGWKKG EQNGQEEKWC EKAVKSLVKK LKKTGQLDEL EKAITTQNVN TKCITIPRSL DGRLQVSHRK GLPHVIYCRL WRWPDLHSHH ELRAMELCEF AFNMKKDEVC VNPYHYQRVE TPVLPPVLVP RHTEIPAEFP PLDDYSHSIP ENTNFPAGIE PQSNIPETPP PGYLSEDGET SDHQMNHSMD AGSPNLSPNP MSPAHNNLDL QPVTYCEPAF WCSISYYELN QRVGETFHAS QPSMTVDGFT DPSNSERFCL GLLSNVNRNA AVELTRRHIG RGVRLYYIGG EVFAECLSDS AIFVQSPNCN QRYGWHPATV CKIPPGCNLK IFNNQEFAAL LAQSVNQGFE AVYQLTRMCT IRMSFVKGWG AEYRRQTVTS TPCWIELHLN GPLQWLDKVL TQMGSPSIRC SSVS
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 500 mM NaCl, pH 7.5.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
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Alternative Name
- SMAD3
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Gene ID
- 4088
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UniProt
- P84022
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Pathways
- Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
Target
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