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SMAD3 Protein (AA 2-425) (DYKDDDDK Tag,His tag)

This Recombinant SMAD3 protein is produced in Escherichia coli (E. coli).
Catalog No. ABIN7314542

Quick Overview for SMAD3 Protein (AA 2-425) (DYKDDDDK Tag,His tag) (ABIN7314542)

Target

See all SMAD3 Proteins
SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Protein Type

Recombinant

Origin

  • 11
  • 3
  • 3
  • 1
  • 1
Human

Source

  • 13
  • 3
  • 1
  • 1
  • 1
Escherichia coli (E. coli)

Application

SDS-PAGE (SDS), Western Blotting (WB), ELISA, Mass Spectrometry (MS)

Purity

Greater than 95 % as determined by reducing SDS-PAGE.
  • Protein Characteristics

    AA 2-425

    Purification tag / Conjugate

    This SMAD3 protein is labelled with DYKDDDDK Tag,His tag.

    Purpose

    Recombinant Human SMAD3 Protein is produced by E.coli expression system and the target gene encoding Ser2-Ser425 is expressed with a 6His, Flag tag at the N-terminus.

    Sequence

    MRGSHHHHHH GSDYKDDDDK SSILPFTPPI VKRLLGWKKG EQNGQEEKWC EKAVKSLVKK LKKTGQLDEL EKAITTQNVN TKCITIPRSL DGRLQVSHRK GLPHVIYCRL WRWPDLHSHH ELRAMELCEF AFNMKKDEVC VNPYHYQRVE TPVLPPVLVP RHTEIPAEFP PLDDYSHSIP ENTNFPAGIE PQSNIPETPP PGYLSEDGET SDHQMNHSMD AGSPNLSPNP MSPAHNNLDL QPVTYCEPAF WCSISYYELN QRVGETFHAS QPSMTVDGFT DPSNSERFCL GLLSNVNRNA AVELTRRHIG RGVRLYYIGG EVFAECLSDS AIFVQSPNCN QRYGWHPATV CKIPPGCNLK IFNNQEFAAL LAQSVNQGFE AVYQLTRMCT IRMSFVKGWG AEYRRQTVTS TPCWIELHLN GPLQWLDKVL TQMGSPSIRC SSVS

    Sterility

    0.2 μm filtered

    Endotoxin Level

    Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
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  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Buffer

    Lyophilized from a 0.2 μm filtered solution of 20  mM PB, 500  mM NaCl,  pH 7.5.

    Handling Advice

    Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

    Storage

    -20 °C

    Storage Comment

    Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
  • Target

    SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

    Alternative Name

    SMAD3

    Gene ID

    4088

    UniProt

    P84022

    Pathways

    Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
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