IL-2 Protein (AA 21-153)

Catalog No. ABIN6253249

This Recombinant IL-2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-2 Protein (AA 21-153) (ABIN6253249)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 21-153

Purpose: IL-2 (human) (rec.) (untagged)

Specificity: The extracellular domain of human IL-2 (aa 21-153) with a N-terminal Met.

Characteristics: Protein. The extracellular domain of human IL-2 (aa 21-153) with a N-terminal Met. Source: E. coli. Endotoxin content: <0.01EU/μg protein (LAL test, Lonza). Lyophilized from sterile 0.1 % TFA, 70 % acetonitrile. Purity: >95 % (SDS-PAGE). Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells, and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils, and induces mononuclear cells to secrete IFN-gamma and TNF-alpha and -beta. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important hemeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens.

Endotoxin Level: <0.01EU/μg protein (LAL test, Lonza).

Biological Activity Comment: Measured in a cell proliferation assay using CTLL2 cells. The ED50 for this effect is typically 0.35-1.4ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from sterile 0.1 % TFA, 70 % acetonitrile.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -80°C

Use & Stability: Stable for at least 1 year after receipt when stored at -80°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2

Background:

Alternate Names/Synonyms: Interleukin-2, Aldesleukin

Product Description: Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells, and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils, and induces mononuclear cells to secrete IFN-gamma and TNF-alpha and -beta. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important hemeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens.

Molecular Weight: ~14kDa (SDS-PAGE)

NCBI Accession: NP_000577

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation