IL-21 Protein (AA 18-146) (Fc Tag)

Catalog No. ABIN6253260

This Recombinant IL-21 protein is produced in HEK-293 Cells.

Quick Overview for IL-21 Protein (AA 18-146) (Fc Tag) (ABIN6253260)

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: >98 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 18-146

Purification tag / Conjugate: This IL-21 protein is labelled with Fc Tag.

Purpose: IL-21 (mouse):Fc (mouse) (rec.)

Specificity: The extracellular domain of mouse IL-21 (aa 18-146) is fused to the N-terminus of the Fc region of mouse IgG2a.

Characteristics: Protein. The extracellular domain of mouse IL-21 (aa 18-146) is fused to the N-terminus of the Fc region of mouse IgG2a. Source: HEK 293 cells. Endotoxin content: <5EU/mg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >98 % (SDS-PAGE). Interleukin-21 (IL-21) is a key factor in the transition between innate and adaptive immune responses secreted by activated T cells. The IL-21 receptor (IL-21R) is expressed in lymphoid tissue, in particular by NK, B, T and dendritic cells, macrophages and endothelial cells. Recent evidence suggests that IL-21 plays a supportive role in the proliferation of T and B cells and influences the cytolytic activity of natural killer cells. IL-21 has been shown to up-regulate genes associated with innate immunity and to inhibit the differentiation of naive T helper cells. IL-21 specifically inhibits IFN-gamma production from developing TH1 cells and is preferentially expressed by TH2 cells. Furthermore IL-21 has been identified as a growth and survival factor for human myeloma cells. IL-21/IL-21R interactions have a unique role in sequentially activating both innate and adaptive immune responses against poorly immunogenic tumors, leading to tumor rejection that is perforin dependent but IFN-gamma independent.

Endotoxin Level: <5EU/mg protein (LAL test, Lonza).

Biological Activity Comment: Measured in a cell proliferation assay using N1186 human T cells.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Alternative Name: IL-21

Background:

Alternate Names/Synonyms: Interleukin-21

Product Description: Interleukin-21 (IL-21) is a key factor in the transition between innate and adaptive immune responses secreted by activated T cells. The IL-21 receptor (IL-21R) is expressed in lymphoid tissue, in particular by NK, B, T and dendritic cells, macrophages and endothelial cells. Recent evidence suggests that IL-21 plays a supportive role in the proliferation of T and B cells and influences the cytolytic activity of natural killer cells. IL-21 has been shown to up-regulate genes associated with innate immunity and to inhibit the differentiation of naive T helper cells. IL-21 specifically inhibits IFN-gamma production from developing TH1 cells and is preferentially expressed by TH2 cells. Furthermore IL-21 has been identified as a growth and survival factor for human myeloma cells. IL-21/IL-21R interactions have a unique role in sequentially activating both innate and adaptive immune responses against poorly immunogenic tumors, leading to tumor rejection that is perforin dependent but IFN-gamma independent.

Molecular Weight: ~45kDa (SDS-PAGE)

NCBI Accession: NP_068554

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response