Prothrombin Protein (His tag)
Quick Overview for Prothrombin Protein (His tag) (ABIN6303199)
Target
See all Prothrombin (F2) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This Prothrombin protein is labelled with His tag.
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Purpose
- Rat Coagulation Factor II, Thrombin / Prothrombin (F2) Protein
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Sequence
- SRGSKENLSP PLGECLLERG RLYQGNLAVT TLGSPCLAWD SLPTKTLSKY QNFDPEVKLV QNFCRNPDRD EEGAWCFVAQ QPGFEYCSLN YCDEAVGEEN HDGDESIAGR TTDAEFHTFF DER
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Biological Activity Comment
- Not tested
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Coagulation Factor II (thrombin) (F2) (AA 328-622) protein
F2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % SDS
Coagulation Factor II (thrombin) (F2) (Active) protein (His tag)F2 Origin: Mouse Host: HEK-293 Cells Recombinant > 96 % as determined by reducing SDS-PAGE. Active
Coagulation Factor II (thrombin) (F2) (AA 1-622) protein (GST tag)F2 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 1 mM DTT, 5 % Trehalose and Proclin-300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))
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Alternative Name
- Coagulation Factor II
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Molecular Weight
- Calculated MW: 15.4 kDa
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UniProt
- P18292
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Pathways
- Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling
Target
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