Gelsolin Protein (GSN) (His tag)

Catalog No. ABIN2940723

This Recombinant Gelsolin protein is produced in Escherichia coli (E. coli).

Quick Overview for Gelsolin Protein (GSN) (His tag) (ABIN2940723)

Target: Gelsolin (GSN)

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Purification tag / Conjugate: This Gelsolin protein is labelled with His tag.

Purpose: Rat Gelsolin (GSN) Protein

Sequence: AAQHGMDDD GTGQKQIWRI EGSNKVLVDP ATYGQFYGGD SYIILYNYRH GGRQGQIIYN WQGAQSTQDE VAASAILTAQ LDEELGGTPV QSRVVQGKEP AHLMSLFGGK PMIIYKGGTS RDGGQTTPAS TRLFQVRASS SGATRAVEVM PKAGALNSND AFVLKTPSAA YLWVGTGASD AEKTGALELL KVLRAQHVQV EEGSEPDGFW EALGGKTAYR TSPRLKDKKM DAHPPRLFAC SNRIGRFVIE EVPGELMQED LATDDVMLLD TWDQVFVWVG KDSQEEEKTE ALTSAKRYIE TDPANRDRRT PITVVRQGFE PPSFVGWFLG WDDDYWSVDP LDRALAELAA

Biological Activity Comment: Not tested

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.

Buffer: Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 1 mM DTT, 5 % Trehalose and Proclin-300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: Gelsolin (GSN)

Alternative Name: Gelsolin

Molecular Weight: Calculated MW: 39.6 kDa
Observed MW: 43 kDa

UniProt: Q68FP1

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, Autophagy