VGF Protein (His tag)

Catalog No. ABIN6126899

This Recombinant VGF protein is produced in Escherichia coli (E. coli).

Quick Overview for VGF Protein (His tag) (ABIN6126899)

Target: VGF (VGF Nerve Growth Factor Inducible (VGF))

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB)

Purity: > 95 %

Product Details

Purification tag / Conjugate: This VGF protein is labelled with His tag.

Purpose: Rat Neurosecretory Protein VGF (VGF) Protein

Sequence: RLEGSFL GGSEAGERLL QQGLAQVEAG RRQAEATRQA AAQEERLADL ASDLLLQYLL QGGARQRDLG GRGLQETQQE RENEREEEAE QERRGGGEDE VGEEDEEAAE AEAEAEEAER ARQNALLFAE EEDGEAGAED KRSQEEAPGH RRKDAEGTEE GGEEDDDDEE MDPQTIDSLI ELSTKLHLPA DDVVSIIEEV EEKRKRKKNA PPEPVPPPRA APAPTHVRSP QPPPPAPARD ELPDWNEVLP PWDREEDEVF PPGPYHPFPN YIRPRTLQPP ASSRRRHFHH ALPPAR

Biological Activity Comment: Not tested

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.

Buffer: Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 5 % Trehalose.

Storage: 4 °C,-80 °C

Storage Comment: Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: VGF (VGF Nerve Growth Factor Inducible (VGF))

Alternative Name: VGF Nerve Growth Factor Inducible

Molecular Weight: Calculated MW: 36.4 kDa
Observed MW (SDS-PAGE): 43 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.

UniProt

P20156

Pathways: Hormone Transport, Carbohydrate Homeostasis