AKR1C4 Protein (AA 1-323)
Quick Overview for AKR1C4 Protein (AA 1-323) (ABIN6387734)
Target
See all AKR1C4 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
-
-
Protein Characteristics
- AA 1-323
-
Sequence
- MDPKYQRVEL NDGHFMPVLG FGTYAPPEVP RNRAVEVTKL AIEAGFRHID SAYLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCTFF QPQMVQPALE SSLKKLQLDY VDLYLLHFPM ALKPGETPLP KDENGKVIFD TVDLSATWEV MEKCKDAGLA KSIGVSNFNC RQLEMILNKP GLKYKPVCNQ VECHPYLNQS KLLDFCKSKD IVLVAHSALG TQRHKLWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIREN IQVFEFQLTS EDMKVLDGLN RNYRYVVMDF LMDHPDYPFS DEY
-
Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
-
Biological Activity Comment
- Specific activity is > 700pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.
-
-
Want other Options for this Protein ?
!Discover Our Predefined Custom Proteins and Custom Protein Services!ProductExpression SystemConjugateOriginPrice starts atExpression System HEK-293 CellsConjugate His tagOrigin HumanPrice starts at $12,595.45Expression System Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin HumanPrice starts at $18,117.43Your project requires further customization? Contact us and discover our custom protein solutions
-
Aldo-keto Reductase Family 1 Member C4 (AKR1C4) (AA 1-323) protein (His tag)
custom-made AKR1C4 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Aldo-keto Reductase Family 1 Member C4 (AKR1C4) (AA 1-343) protein (His-IF2DI Tag)AKR1C4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Aldo-keto Reductase Family 1 Member C4 (AKR1C4) (AA 1-323) protein (GST tag)AKR1C4 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Aldo-keto Reductase Family 1 Member C4 (AKR1C4) protein (Myc-DYKDDDDK Tag)AKR1C4 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Comment
-
Bioactivity Validated
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- 1 mg/mL
-
Buffer
- Liquid. In 20 mM Tris-HCl buffer( pH 8.5) containing 0.1M NaCl, 10 % glycerol, 1 mM DTT
-
Storage
- 4 °C,-20 °C,-80 °C
-
Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
-
-
- AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))
-
Alternative Name
- Aldo-keto Reductase 1C4/AKR1C4
-
Background
- AKR1C1 also known as Aldo-keto reductase family 1 member C4, belongs to aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. It catalyzes the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. Recombinant human AKR1C4 protein was expressed in E. coli and purified by using conventional chromatography.
-
Molecular Weight
- 37 kDa (323aa)
-
NCBI Accession
- NP_001809
-
UniProt
- P17516
-
Pathways
- Steroid Hormone Biosynthesis
Target
-
