UBE2I Protein (AA 1-158)

Catalog No. ABIN666677

This Recombinant UBE2I protein is produced in Escherichia coli (E. coli).

Quick Overview for UBE2I Protein (AA 1-158) (ABIN666677)

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-158

Sequence: MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA QAKKFAPS

Characteristics: Ubc9 (Human ubiquitin conjugating enzyme 9) Human, Recombinant, expressed in E.coli

Purification: > 95% by SDS-PAGE

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 50 mM HEPES buffer ( pH 7.4) containing 150 mM NaCl, 1 mM DTT, 10 % glycerol

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Alternative Name: Ubc9/UBE2I

Background: Human ubc9 is homologous to ubiquitin-conjugating enzymes (E2s). However, instead of conjugating ubiquitin, it conjugates a ubiquitin homologue, small ubiquitin-like modifier 1 (SuMO-1). And hubc9 retains striking structural and functional conservation with yeast ubc9. The ubiquitin-dependent protein degradation system has been recognized as a complete enzymatic pathway that is responsible for the selective degradation of abnormal and short-lived proteins. The conjugation of ubiquitin reuires the activities of ubiquitin-activating (E1) and -conjugating (E2) enzymes.

Molecular Weight: 18 kDa (158aa) confirmed by MALDI-TOF

NCBI Accession: NP_003336

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway