Dihydrofolate Reductase Protein (DHFR) (AA 1-187) (His tag)
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- Target See all Dihydrofolate Reductase (DHFR) Proteins
- Dihydrofolate Reductase (DHFR)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-187
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Dihydrofolate Reductase protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- DHFR, 1-187 aa, Human, His-tagged, Recombinant, E.coli
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product DHFR Protein
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Dihydrofolate Reductase (DHFR) (AA 1-187) protein (His tag)
DHFR Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Dihydrofolate Reductase (DHFR) (AA 1-187) protein (GST tag)DHFR Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Dihydrofolate Reductase (DHFR) (AA 2-187) protein (His-SUMO Tag)DHFR Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Dihydrofolate Reductase (DHFR) protein (Myc-DYKDDDDK Tag)DHFR Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M Nacl 2mM DTT, and 30% glycerol
- Storage
- 4 °C
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- Target
- Dihydrofolate Reductase (DHFR)
- Alternative Name
- DHFR (DHFR Products)
- Background
- DHFR, also known as Dihydrofolate reductase, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Recombinant Dihydrofolate reductase protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Dihydrofolate reductase, EC 1.5.1.3. NCBI no.: NP_000782.1
- Molecular Weight
- 23.6 kDa (207aa), confirmed by MALDI-TOF.
- Pathways
- Mitotic G1-G1/S Phases
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