GST Protein (AA 1-224) (His tag)

Catalog No. ABIN666898

Recombinant GST protein expressed in Escherichia coli (E. coli).

Quick Overview for GST Protein (AA 1-224) (His tag) (ABIN666898)

Target: GST (Glutathione S Transferase (GST))

Protein Type: Recombinant

Origin: Schistosoma japonicum

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % by SDS - PAGE

Product Details

Protein Characteristics: AA 1-224

Purification tag / Conjugate: This GST protein is labelled with His tag.

Characteristics: GST, 1-224aa, Schistosoma japonicum, His-tag, E.coli

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid. In Phosphate buffered saline (pH7.4), 10% glycerol

Storage: 4 °C

Target Details

Target: GST (Glutathione S Transferase (GST))

Alternative Name: GST

Background: Glutathione S-transferase (GST) represents a major group of detoxification enzymes. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. Glutathione S-transferase was originally separated from Schistosoma japonicum but currently isolated from recombinant E.coli source. Recombinant human GST, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Sj26 antigen, SjGST, Glutathione S-Transferase class-mu 26 kDa isozyme Glutathione S Transferase. NCBI no.: P08515

Molecular Weight: 28.3 kDa (244aa), confirmed by MALDI-TOF.