CD40 Ligand Protein (CD40LG)

Catalog No. ABIN6699579

Supplier Product No.: 009-001-u68-0050

Recombinant CD40 Ligand protein expressed in Escherichia coli (E. coli).

Quick Overview for CD40 Ligand Protein (CD40LG) (ABIN6699579)

Target: CD40 Ligand (CD40LG)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Product Details

Supplier Product No.: 009-001-u68-0050

Supplier: Rockland

Purpose: Human CD40 Ligand Recombinant Protein

Purification: CD40 Ligand purity was determined to be greater than 98% as determined by analysis by HpLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is tested by HEK-blue ™ CD40L Activity. Acceptable Level <50ng/mL, >2.0x10^4 units/mg. Additionally testing by ELISA: IL-8 production by human PBMCs.

Application Details

Application Notes:

Application Note: CD40 Ligand Recombinant Protein has been tested by SDS-PAGE and biological assay and is suitable as a control for polyclonal or monoclonal anti-CD40 Ligand in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 50μL

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40LG

Background:

Synonyms: TNFSF5, TRAP, CD154, gp39, T-BAM

Background: CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-α, and IL-2. Recombinant human CD40 Ligand is a non-glycosylated protein, containing 149 amino acids, with a molecular weight of 16.3 kDa.

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints