CCL13 Protein

Catalog No. ABIN6699596

Supplier Product No.: 009-001-v77-0005

This Recombinant CCL13 protein is expressed in Escherichia coli (E. coli).

Quick Overview for CCL13 Protein (ABIN6699596)

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Product Details

Supplier Product No.: 009-001-v77-0005

Supplier: Rockland

Purpose: Human Monocyte Chemotactic Protein-4 (CCL13) Recombinant Protein

Purification: Monocyte Chemotactic protein-4 (CCL13) purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The biological activity is determined by the ability of MCP-4 to chemoattract human monocytes and is typically between 7-75 ng/mL.

Application Details

Application Notes:

Application Note: Monocyte Chemotactic Protein-4 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Monocyte Chemotactic Protein-4 in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Alternative Name: CCL13

Background:

Synonyms: CK-beta-10, Monocyte chemoattractant protein 4, Monocyte chemotactic protein 4 (MCP-4), NCC-1, Small-inducible cytokine A13

Background: Monocyte Chemotactic Protein 4 (MCP-4), also called CCL13, is induced by inflammatory proteins such as IL-1 and TNFα. MCP-4 is a ligand for three different G protein coupled receptors, CCR2, CCR3 and CCR5. MCP-4 activates signaling in monocytes, T lymphocytes, eosinophils and basophils and this signaling is associated with the allergic response. Recombinant human MCP-4 is a non-glycosylated protein, containing 74 amino acids, with a molecular weight of 8.5 kDa.

UniProt: Q99616

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, The Global Phosphorylation Landscape of SARS-CoV-2 Infection