CCL18 Protein

Catalog No. ABIN6699605

Supplier Product No.: 009-001-w00-0010

This Recombinant CCL18 protein is expressed in Escherichia coli (E. coli).

Quick Overview for CCL18 Protein (ABIN6699605)

Target: CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Product Details

Supplier Product No.: 009-001-w00-0010

Supplier: Rockland

Purpose: Human Macrophage Inflammatory Protein-4 (CCL18) Recombinant Protein

Purification: Macrophage Inflammatory protein-4 (CCL18) purity was determined to be greater than 98% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the ability to chemoattract human primary T cells or PBMCs and is typically 1.0-8.0 ng/mL.

Application Details

Application Notes:

Application Note: Macrophage Inflammatory Protein-4 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Macrophage Inflammatory Protein-4 in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))

Alternative Name: CCL18

Background:

Synonyms: PARC, Alternative macrophage activation-associatedCC chemokine 1 (AMAC-1), Dendritic cell chemokine 1 (DC-CK1), Macrophage inflammatory protein 4 (MIP-4), Pulmonary andactivation-regulated chemokine, Small-inducible cytokine A18

Background: Macrophage Inflammatory Protein-4 (MIP-4), also called CCL18, is a chemokine expressed in the lymph nodes, lungs, placenta and bone marrow. Although the MIP-4's receptor remains undetermined, MIP-4 is known to act as a chemoattractant for activated and non-activated T cells. Recombinant human MIP-4 is a non-glycosylated protein, containing 69 amino acids, with a molecular weight of 7.8 kDa.

UniProt: P55774