CCL2 Protein

Catalog No. ABIN6699615

Supplier Product No.: 012-001-v74-0100

Recombinant CCL2 protein expressed in Escherichia coli (E. coli).

Quick Overview for CCL2 Protein (ABIN6699615)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Product Details

Supplier Product No.: 012-001-v74-0100

Supplier: Rockland

Purpose: Rat Monocyte Chemotactic Protein-1 (CCL2) Recombinant Protein

Purification: Monocyte Chemotactic protein-1 (CCL2) purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the ability to chemoattract human monocytes or THP-1 cells at 4 - 15 ng/mL.

Application Details

Application Notes:

Application Note: Monocyte Chemotactic Protein-1 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Monocyte Chemotactic Protein-1 in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: Ccl2

Background:

Synonyms: Immediate-early serum-responsive protein JE, Monocyte chemoattractant protein 1, MCAF, Monocyte chemotactic protein 1 (MCP-1), Small-inducible cytokine A2

Background: Monocyte Chemotactic Protein 1 (MCP-1), also known as CCL2, is thought to be produced by injured or infected tissues. MCP-1 signals through G protein-coupled receptors, CCR2 and CCR4, to recruit memory T cells, monocytes and dendritic cells. Recombinant rat MCP-1is a non-glycosylated protein, containing 125 amino acids, with a molecular weight of 14.1 kDa.

UniProt: P14844

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection