CCL3 Protein
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- Target See all CCL3 Proteins
- CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Supplier Product No.
- 009-001-315
- Supplier
- Rockland
- Purpose
- MIP-1α Human Recombinant Protein
- Purification
- Purity was determined to be greater than 97% by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.
- Purity
- 97,00%
- Endotoxin Level
- Measured by LAL is 0.15 EU/μg protein.
- Biological Activity Comment
- Determined by its ability to chemoattract human monocytes using a concentration range of 0.5 - 8 ng/ml.
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- Discover our top product CCL3 Protein
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- Application Notes
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Other: User Optimized
Application_Note: MIP-1α protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti--MIP-1α in immunological assays.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 20 μL (20-200 μL)
- Concentration
- 0.1 mg/mL
- Buffer
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
- Preservative
- Without preservative
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Expiry Date
- 6 months
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- Target
- CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
- Alternative Name
- CCL3 (CCL3 Products)
- Background
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Synonyms: Human Macrophage Inflammatory Protein-1, CCL3 alpha cytokine, C-C motif chemokine 3, Small-inducible cytokine A3, MIP-1-alpha, Tonsillar lymphocyte LD78 alpha protein, G0/G1 switch regulatory protein 19-1, SIS-beta, PAT 464.1
Background: Recombinant Human Macrophage Inflammatory Protein-1 (CCL3, MIP-1) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7820 Daltons.
- UniProt
- P10147
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Autophagy
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