CCL3 Protein

Catalog No. ABIN6699630

Supplier Product No.: 012-001-v80-0100

Recombinant CCL3 protein expressed in Escherichia coli (E. coli).

Quick Overview for CCL3 Protein (ABIN6699630)

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Product Details

Supplier Product No.: 012-001-v80-0100

Supplier: Rockland

Purpose: Rat Macrophage Inflammatory Protein-1 alpha (CCL3) Recombinant Protein

Purification: Macrophage Inflammatory protein-1 alpha (CCL3) purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the ability to chemoattract human PBMCs at 1-10 ng/mL.

Application Details

Application Notes:

Application Note: Macrophage Inflammatory Protein-1 alpha Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Macrophage Inflammatory Protein-1 alpha in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Alternative Name: Ccl3

Background:

Synonyms: LD78α, Macrophage inflammatory protein 1-alpha (MIP-1-alpha)

Background: Macrophage Inflammatory Protein-1 alpha (MIP-1α), also known as CCL3, is produced by macrophages and is thought to induce inflammatory responses, including superoxide production by neutrophils. MIP-1α can exist as a naturally occurring heterodimer with MIP-1β and has been shown to have antiviral activity against HSV-1. Recombinant rat MIP-1α is a non-glycosylated protein, containing 69 amino acids, with a molecular weight of 7.8 kDa.

UniProt: P50229

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy