EGF Protein

Catalog No. ABIN6699795

Supplier Product No.: 009-f01-c26-0100

Recombinant EGF protein expressed in Escherichia coli (E. coli).

Quick Overview for EGF Protein (ABIN6699795)

Target: EGF (Epidermal Growth Factor (EGF))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Grade: Animal-Free

Product Details

Supplier Product No.: 009-f01-c26-0100

Supplier: Rockland

Purpose: Human Epidermal Growth Factor Recombinant Protein (Animal Free)

Purification: Epidermal Growth Factor (EDF) is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 95% as determined by analysis by HPLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-PAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of murine BALB/c 3T3 cells and is typically between 20-100 pg/mL.

Application Details

Application Notes:

Application Note: Epidermal Growth Factor Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Epidermal Growth Factor in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 1.0 mL

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF

Background:

Synonyms: Urogastrone, URG

Background: Epidermal Growth Factor (EGF) is a growth factor that stimulates the proliferation of epithelial and epidermal cells. EGF family members are characterized by three intramolecular disulfide bonds and can bind to four different receptor tyrosine kinases known as EGFR/ErbB1, ErbB2, ErbB3, and ErbB4. Recombinant human EGF is a single, non-glycosylated protein containing 53 amino acids, with a molecular weight of 6.2 kDa.

UniProt: P01133

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation