GDF15 Protein

Catalog No. ABIN6699887

Supplier Product No.: 009-001-v09-0020

This Recombinant GDF15 protein is expressed in Escherichia coli (E. coli).

Quick Overview for GDF15 Protein (ABIN6699887)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Product Details

Supplier Product No.: 009-001-v09-0020

Supplier: Rockland

Purpose: Human Growth and Differentiation Factor-15 (D variant) Recombinant Protein

Purification: Growth and Differentiation Factor-15 (D variant) purity was determined to be greater than 95% as determined by analysis by HpLC, UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the inhibition of DU-145 cells and is typically 1-2 μg/mL.

Application Details

Application Notes:

Application Note: Growth and Differentiation Factor-15 Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Growth and Differentiation Factor-15 in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF15

Background:

Synonyms: Macrophage inhibitory cytokine 1 (MIC-1), NSAID-activated gene 1 protein (NAG-1), NSAID-regulated gene 1 protein (NRG-1), Placental TGFβ, Placental bone morphogenetic protein, Prostate differentiation factor

Background: Growth and Differentiation Factor 15 (GDF-15) is a TGFβ family member, made by the placenta and heart tissues, that has a role in regulating inflammatory and apoptotic pathways. GDF-15 has become an immerging marker of early heart disease and has the potential as being used as a molecule for screening patients for early heart failure. Recombinant human GDF-15 D is a non-glycosylated, disulfide linked homodimer, containing two identical 113 amino acid chains, with a total molecular weight of 24.5 kDa. There is a His to an Asp substitution at position 7.

UniProt: Q99988

Pathways: SARS-CoV-2 Protein Interactome