GM-CSF Protein

Catalog No. ABIN6699894

Supplier Product No.: 009-001-314

Recombinant GM-CSF protein expressed in Escherichia coli (E. coli).

Quick Overview for GM-CSF Protein (ABIN6699894)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Product Details

Supplier Product No.: 009-001-314

Supplier: Rockland

Purpose: GM-CSF Human Recombinant Protein

Purification: Recombinant protein corresponding to amino acids 18 to 144 of mature human GM-CSF with addition of methionine on the N-terminus. Purity is greater than 98% as determined by reducing and non-reducing SDS-PAGE and by analytical RP-HPLC, each against a known standard.

Endotoxin Level: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: Human GM-CSF is fully biologically active when compared to standard. The ED50, as determined by the dose-dependent induction of human TF-1 cell proliferation, is 0.14-0.2 ng/ml.

Application Details

Application Notes:

Application Note: GM-CSF protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-GM-CSF in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: CSF2

Background:

Synonyms: Granulocyte Macrophage Colony Stimulating Factor, Granulocyte Macrophage-CSF, Granulocyte-macrophage colony-stimulating factor, GM-CSF cytokine, Colony-stimulating factor, CSF, CSF2, Sargramostim, Molgramostin

Background: Granulocyte Macrophage Colony Stimulating Factor is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors , including IL2-beta, IL-3 , IL -6 , IL-7 , EPO and the Prolactin receptors. Recombinant Human Granulocyte Macrophage CSF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular weight of 14.5 kDa.

UniProt: P04141

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin