GM-CSF Protein

Catalog No. ABIN6699900

Supplier Product No.: 009-f01-v11-0100

Recombinant GM-CSF protein expressed in Escherichia coli (E. coli).

Quick Overview for GM-CSF Protein (ABIN6699900)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Grade: Animal-Free

Product Details

Supplier Product No.: 009-f01-v11-0100

Supplier: Rockland

Purpose: Human Granulocyte Macrophage Colony Stimulating Factor Recombinant Protein (Animal Free)

Purification: Granulocyte Macrophage Colony Stimulating Factor is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-PAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent induction of human TF-1 cell proliferation and is typically less than 0.1 ng/mL.

Application Details

Application Notes:

Application Note: Granulocyte Macrophage Colony Stimulating Factor Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Granulocyte Macrophage Colony Stimulating Factor in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: CSF2

Background:

Synonyms: Molgramostin, Pluripoietin-α, MGI1GM, Sargramostim

Background: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is hematopoietic factor produced by endothelial cells, monocytes, fibroblasts and T cells in response to a number of inflammatory mediators. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. Human and mouse GM-CSF show no cross-reactivity. Recombinant human GM-CSF is a non-glycosylated protein, containing 128 amino acids, with a molecular weight of 14.6 kDa.

UniProt: P04141

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin