IL-13 Protein

Catalog No. ABIN6700028

Supplier Product No.: 010-001-v52-0010

This Recombinant IL-13 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-13 Protein (ABIN6700028)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Product Details

Supplier Product No.: 010-001-v52-0010

Supplier: Rockland

Purpose: Mouse Interleukin-13 Recombinant Protein

Purification: Interleukin-13 purity was determined to be greater than 95% as determined by analysis by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose dependent proliferation of TF-1 cells and is typically less than 4 ng/mL.

Application Details

Application Notes:

Application Note: Interleukin-13 Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Interleukin-13 in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Alternative Name: IL13

Background:

Synonyms: NC30

Background: Interleukin-13 (IL-13) is an important cytokine secreted from Th2 cells. The functions attributed to IL-13 overlap significantly with those of IL-4 (induces IgE secretion from B cells and inhibits expression of inflammatory cytokines such as IL-1β, TNF-α, IL-8 and IL-6), but differs from IL-4 in that IL-13 seems to link inflammatory response of immune cells to the pathophysiological changes in the surrounding non-immune cells. The receptor subunits of IL-13 consist of IL-4Rα, IL-13Rα1 and IL-13Rα2. Human and mouse IL-13 are cross-reactive. Recombinant mouse IL-13 is a non-glycosylated protein, containing 111 amino acids, with a molecular weight of 12.3 kDa.

UniProt: P20109

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport