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IL-2 Protein

This Recombinant IL-2 protein is produced in Escherichia coli (E. coli).
Rockland
Catalog No. ABIN6700063
Supplier Product No.: 009-001-b95

Quick Overview for IL-2 Protein (ABIN6700063)

Target

See all IL-2 (IL2) Proteins
IL-2 (IL2) (Interleukin 2 (IL2))

Protein Type

Recombinant

Origin

  • 46
  • 26
  • 9
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Source

  • 49
  • 29
  • 23
  • 6
  • 4
  • 3
  • 1
  • 1
  • 1
Escherichia coli (E. coli)

Application

SDS-PAGE (SDS)

Purity

90,00%
  • Supplier Product No.

    009-001-b95

    Supplier

    Rockland

    Purpose

    IL-2 Human Recombinant Protein

    Purification

    Purity was determined to be greater than 90% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS- PAGE, against known standard.

    Endotoxin Level

    Measured by LAL is < 0.5 EU/μg protein.

    Biological Activity Comment

    Recombinant Human IL-2 is fully bioactive when compared to standard. The ED50 as measured by the dose-dependent proliferation of CTLL-2 cells is typically 1.0 ng/ml.
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  • Application Notes

    Application Note: IL-2 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-2 in immunological assays.

    Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 50μL

    Concentration

    0.1 mg/mL

    Buffer

    Buffer: 0.1 % Trifluoroacetic acid

    Stabilizer: None

    Preservative

    Without preservative

    Storage

    -20 °C

    Storage Comment

    Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

    Expiry Date

    6 months
  • Target

    IL-2 (IL2) (Interleukin 2 (IL2))

    Alternative Name

    IL2

    Background

    Synonyms: Interleukin-2 cytokine, aldesleukin, T cell growth factor2, TCGF,

    Background: Interleukin-2 (IL2) is a secreted immunomodulatory cytokine that is essential in regulation and proliferation of T and B lymphocytes, and other activities crucial to regulation of the immune response. The receptor of this cytokine (the IL-2R receptor) is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells. Produced by T-cells in response to antigenic or mitogenic stimulation, this protein can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. Recombinant IL-2 produced in E. coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 15,517 Daltons. Recombinant human IL-2 has a Ser substitute for Cysteine at position 126.

    UniProt

    Q6QWN0

    Pathways

    JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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