IL-2 Protein

Catalog No. ABIN6700064

Supplier Product No.: 009-f01-v27-0010

This Recombinant IL-2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-2 Protein (ABIN6700064)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 90,00%

Grade: Animal-Free

Product Details

Supplier Product No.: 009-f01-v27-0010

Supplier: Rockland

Purpose: Human Interleukin-2 Recombinant Protein (Animal Free)

Purification: Interleukin-2 is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 90% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-PAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is measured by the dose-dependent proliferation of CTLL-2 cells and is typically less than 1 ng/mL.

Application Details

Application Notes:

Application Note: Human Interleukin-2 Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-Human Interleukin-2 in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL2

Background:

Synonyms: T cell growth factor, TCGF, Aldesleukin

Background: Interleukin-2 (IL-2) is an immuno-modulatory cytokine produced by stimulated T cells. IL-2 signals through the IL-2R receptor to induce proliferation of activated T cells. IL-2 also stimulates proliferation and differentiation of B cells, natural killer cells, monocytes and macrophages. Recombinant human IL-2 is a non-glycosylated protein, containing 134 amino acids, with a molecular weight of 15.5 kDa. Recombinant human IL-2 has a Ser substitute for Cysteine at position 126.

UniProt: P60568

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation