IL-2 Protein

Catalog No. ABIN6700068

Supplier Product No.: 010-001-v27-0020

Recombinant IL-2 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-2 Protein (ABIN6700068)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Product Details

Supplier Product No.: 010-001-v27-0020

Supplier: Rockland

Purpose: Mouse Interleukin-2 Recombinant Protein

Purification: Interleukin-2 purity was determined to be greater than 98% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by its ability to induce the proliferation of CTLL-2 cells and is typically 0.1-0.4 ng/mL.

Application Details

Application Notes:

Application Note: Mouse Interleukin-2 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Mouse Interleukin-2 in immunological assays. Lyophilized in 0.01M sodium citrate, pH 4.0.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Buffer: Lyophilized in 0.01M sodium citrate, pH 4.0.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL2

Background:

Synonyms: T cell growth factor, TCGF, Aldesleukin

Background: Interleukin-2 (IL-2) is an immuno-modulatory cytokine produced by stimulated T cells. IL-2 signals through the IL-2R receptor to induce proliferation of activated T cells. IL-2 also stimulates proliferation and differentiation of B cells, natural killer cells, monocytes and macrophages. Recombinant mouse IL-2 is a non-glycosylated protein, containing 149 amino acids, with a molecular weight of 17.2 kDa.

UniProt: P04351

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation