IL-21 Protein

Catalog No. ABIN6700085

Supplier Product No.: 010-001-v61-0100

This Recombinant IL-21 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-21 Protein (ABIN6700085)

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Product Details

Supplier Product No.: 010-001-v61-0100

Supplier: Rockland

Purpose: Mouse Interleukin-21 Recombinant Protein

Purification: Interleukin-21 purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by a dose-dependent proliferation of lymphocytes co-stimulated with anti-CD3ε and is typically 2-50 ng/mL.

Application Details

Application Notes:

Application Note: Interleukin-21 Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-21 in immunological assays. Lyophilized in 20 mM sodium bicarbonate, pH 8.5.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer: Lyophilized in 20 mM sodium bicarbonate, pH 8.5.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Alternative Name: Il21

Background:

Synonyms: Interleukin-21, IL-21, Za11, Interleukin 23

Background: Interleukin-21 (IL-21) is a member of the common gamma-chain family of cytokines (includes IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15) and is expressed by activated CD4+ T cells. IL-21 has a private receptor known as IL-21R, which is expressed on T, B, and NK cells. Within the B cell lineage, IL-21 regulates IgG1 production and cooperates with IL-4 for the production of multiple Ab classes in vivo. Initial studies have demonstrated that IL-21 has pleiotropic effects on the proliferation, differentiation, and effector functions of B, T, natural killer, and dendritic cells. Recombinant mouse IL-21 is a non-glycosylated protein, containing 130 amino acids, with a total molecular weight of 15.1 kDa.

UniProt: Q9ES17

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response