IL-5 Protein

Catalog No. ABIN6700150

Supplier Product No.: 010-001-v42-0100

This Recombinant IL-5 protein is produced in Escherichia coli (E. coli).

Quick Overview for IL-5 Protein (ABIN6700150)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 97,00%

Product Details

Supplier Product No.: 010-001-v42-0100

Supplier: Rockland

Purpose: Mouse Interleukin-5 Recombinant Protein

Purification: Interleukin-5 purity was determined to be greater than 97% as determined by HpLC and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent induction of TF-1 cell proliferation and is typically less than 2 ng/mL.

Application Details

Application Notes:

Application Note: Interleukin-5 Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-Interleukin-5 in immunological assays. Lyophilized in 20 mM sodium bicarbonate, pH 9.0.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer: Lyophilized in 20 mM sodium bicarbonate, pH 9.0.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: Il5

Background:

Synonyms: EDF, B-cell growthfactor II (BCDFII), Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor (TRF)

Background: Interleukin-5 (IL-5) is a hematopoietic growth factor expressed in Th2, mast cells and eosinophils. IL-5 acts through its receptor, the IL-5 receptor (IL-5R) and is involved in B cell growth and eosinophil activation. IL-5 has been shown to be and is regulated by GATA-3, in addition to other transcription factors. Human and mouse IL-5 are cross-reactive. Recombinant mouse IL-5 is a non-glycosylated, disulfide-linked homodimer, containing two 113 amino acid chains, with a total molecular weight of 26.2 kDa.

UniProt: P04401

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour