IL-6 Protein

Catalog No. ABIN6700152

Supplier Product No.: 009-001-310

Recombinant IL-6 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-6 Protein (ABIN6700152)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Product Details

Supplier Product No.: 009-001-310

Supplier: Rockland

Purpose: IL-6 Human Recombinant Protein

Purification: Purity greater than 95% as determined by analysis of RP-HPLC and reducing and non-reducing SDS-PAGE against a known standard.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: IL-6 activity is determined by the dose-dependant stimulation of mouse B9 cells and is typcially less than 1 ng/mL.

Application Details

Application Notes:

Application Note: For easiest restoration, it is recommended to restore with 0.2 mL of sterile water for a stock concentration of 0.1 mg/mL. Interleukin-6 (IL-6) protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Interleukin-6 (IL-6) in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 200μL

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

For easiest restoration, it is recommended to restore with 0.2 mL of sterile water for a stock concentration of 0.1 mg/mL.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL6

Background:

Synonyms: Interleukin-6 cytokine, IL-6, B-cell stimulatory factor 2, BSF-2, Interferon beta-2, IFN-beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, Interleukin HP-1

Background: Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contains four conserved cysteine residues involved in two disulfide bonds. They have a compact, globular fold (similar to other interleukins), stabilized by the 2 disulfide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist: the helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. The fourth alpha-helix is important to the biological activity of the molecule. Interleukin (IL)-6 is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. Recombinant Human IL-6 produced in E.coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 21,000 Daltons.

UniProt: P05231

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome