C-JUN Protein (GST tag)

Catalog No. ABIN6700182

Supplier Product No.: 009-001-r71s

Recombinant C-JUN protein expressed in Escherichia coli (E. coli).

Quick Overview for C-JUN Protein (GST tag) (ABIN6700182)

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >50%

Product Details

Purification tag / Conjugate: This C-JUN protein is labelled with GST tag.

Supplier Product No.: 009-001-r71s

Supplier: Rockland

Purpose: c-JUN recombinant protein-GST fusion protein

Purification: Recombinant human c-JUN (1-79) expressed in E. coli cells using an N-Terminal GST Protein. The purity was determined to be >50% by densitometry.

Application Details

Application Notes:

Application Note: c-JUN Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. c-JUN Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~ 35 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Other: Kinase Assay-User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: c-JUN Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Alternative Name: JUN

Background:

Synonyms: AP1, Transcription factor AP-1, Activator protein 1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39

Background: c-Jun is a proto-oncogene that forms a complex with c-Fos which regulates transcription from promoters containing AP-1 activation elements (1). c-Jun has specific DNA binding activity and following in vitro translation, c-Jun binds as a homodimer to the AP-1 DNA site. The transactivating function of c-Jun is acutely regulated by a wide variety of cellular signals via modulation of phosphorylation of two serines (63 and 73). Jun N-terminal domain kinases (JNKs), are responsible for mediating S63/73 phosphorylation on c-Jun in response to a variety of cellular stimuli including TNF-a, heat stress and u.v. light (2). c-JUN Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, Apoptosis/Autophagy, Cardiovascular Disease, Cellular Stress, Inflammation, JNK/SAPK Pathway, Neurobiology, and NfkB Pathway research.

NCBI Accession: NM_002228

Pathways: MAPK Signaling, RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins