NGFB Protein

Catalog No. ABIN6700287

Supplier Product No.: 009-001-w03-0020

Recombinant NGFB protein expressed in Escherichia coli (E. coli).

Quick Overview for NGFB Protein (ABIN6700287)

Target: NGFB (Nerve Growth Factor beta (NGFB))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 97,00%

Product Details

Supplier Product No.: 009-001-w03-0020

Supplier: Rockland

Purpose: Human Nerve Growth Factor beta Recombinant Protein

Purification: Nerve Growth Factor beta purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the ability to stimulate proliferation of TF-1 cells and is typically less than 1 ng/mL.

Application Details

Application Notes:

Application Note: Nerve Growth Factor beta Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-Nerve Growth Factor beta in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: NGFB (Nerve Growth Factor beta (NGFB))

Alternative Name: NGF beta

Background:

Synonyms: β-NGF

Background: Nerve Growth Factor (NGF-β) is a neurotrophic factor related to BDNF, NT-3 and NT-4. NGF-β acts through its receptor β-NGFR, and is involved in the development and maintenance of the sensory and sympathetic nervous systems. NGF-β is also involved in the growth, differentiation and survival of B lymphocytes. Human, mouse and rat proteins show cross-reactivity. Recombinant human NGF-β is a non-covalently linked homodimer, containing two 121 amino acid chains, with a total molecular weight of 27.2 kDa.

UniProt: P01138

Pathways: NF-kappaB Signaling, RTK Signaling, Regulation of Cell Size