NGFB Protein (AA 122-239)

Catalog No. ABIN7319222

Recombinant NGFB protein expressed in HEK-293 Cells.

Quick Overview for NGFB Protein (AA 122-239) (ABIN7319222)

Target: NGFB (Nerve Growth Factor beta (NGFB))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Cell Culture (CC)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 122-239

Purpose: Recombinant Human β-NGF/NGFB Protein (aa 122-239, Human Cells)

Sequence: Ser122-Arg239

Characteristics: Recombinant Human beta-Nerve Growth Factor is produced by our Mammalian expression system and the target gene encoding Ser122-Arg239 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.04-0.4 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, pH 7.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: NGFB (Nerve Growth Factor beta (NGFB))

Alternative Name: beta-NGF/NGFB

Background: Beta-NGF,Beta-Nerve Growth Factor,NGF,NGFB,Human β-Nerve Growth Factor (β-NGF) was initially isolated in the mouse submandibular gland. It is composed of three non-covalently linked subunits α, β, and γ, it exhibits all the biological activities ascribed to NGF. It is structurally related to BDNF, NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. Β-NGF is a neurotrophic factor that signals through its receptor β-NGF, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. These results suggest that β-NGF is a pleiotropic cytokine, which in addition to its neurotropic activities may have an important role in the regulation of the immune system. Human β-NGF shares 90 % sequence similarity with mouse protein and shows cross-species reactivity.

Molecular Weight:

Calculated MW: 13.3 kDa

Observed MW: 14 kDa

Gene ID: 4803

UniProt: P01138

Pathways: NF-kappaB Signaling, RTK Signaling, Regulation of Cell Size