Chemerin Protein

Catalog No. ABIN6700482

Supplier Product No.: 009-001-u70-0100

Recombinant Chemerin protein expressed in Escherichia coli (E. coli).

Quick Overview for Chemerin Protein (ABIN6700482)

Target: Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Product Details

Supplier Product No.: 009-001-u70-0100

Supplier: Rockland

Purpose: Human Chemerin Recombinant Protein

Purification: Chemerin purity was determined to be greater than 98% as determined by analysis by HpLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by its ability to chemoattract human Chem23R transfected BaF3 mouse pro-B cells and is typically 4-20 ng/mL.

Application Details

Application Notes:

Application Note: Chemerin Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Chemerin in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))

Alternative Name: RARRES2

Background:

Synonyms: Tazarotene-induced gene 2 (TIG2), RARRES2

Background: Chemerin is a chemoattractant expressed in white adipose, liver and lung tissues. Chemerin is a ligand for the G-protein coupled receptor known as ChemR23 (or chemokine-like receptor-1), which is expressed mainly on dendritic cells, macrophages and some adipocytes. Recombinant human Chemerin is a non-glycosylated protein, containing 138 amino acids, with a total molecular weight of 16 kDa.

UniProt: Q99969

Pathways: Brown Fat Cell Differentiation