SMAD3 Protein (GST tag)

Catalog No. ABIN6700547

Supplier Product No.: 009-001-t39s

This Recombinant SMAD3 protein is expressed in Escherichia coli (E. coli).

Quick Overview for SMAD3 Protein (GST tag) (ABIN6700547)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >90%

Product Details

Purification tag / Conjugate: This SMAD3 protein is labelled with GST tag.

Supplier Product No.: 009-001-t39s

Supplier: Rockland

Purpose: SMAD3 recombinant protein-GST fusion protein

Purification: Recombinant full-length human SMAD3 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Application Details

Application Notes:

Application Note: SMAD3 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. SMAD3 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~77 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Other: Kinase Assay-User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: SMAD3 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: SMAD3

Background:

Synonyms: MADH3, JV15-2, HSPC193, HsT17436, MGC60396, DKFZP586N0721, DKFZp686J10186, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2

Background: SMAD3 is a direct mediator of transcriptional activation by the TGFβ receptor. The activity of SMAD3 is regulated by the TGFβ receptors, and SMAD3 is phosphorylated and associated with the ligand-bound receptor complex. TGFβ stimulation leads to phosphorylation and activation of SMAD3, which form a complex with SMAD4 that accumulate in the nucleus and regulate transcription of target genes such as CDK inhibitor (1). SMAD3 containing a C-terminal truncation acts as a dominant-negative inhibitor of the normal TGFβ response. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2 (2). SMAD3 Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, AKT/PKB Pathway, Angiogenesis, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Inflammation, JAK/STAT Pathway, JNK/SAPK Pathway, NfkB Pathway, and WNT Signaling research.

NCBI Accession: NM_005902

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy