TWF1 Protein (GST tag)

Catalog No. ABIN6700770

Supplier Product No.: 009-001-t10s

Recombinant TWF1 protein expressed in Insect cells (Sf9).

Quick Overview for TWF1 Protein (GST tag) (ABIN6700770)

Target: TWF1 (Twinfilin, Actin-Binding Protein 1 (TWF1))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >95%

Product Details

Purification tag / Conjugate: This TWF1 protein is labelled with GST tag.

Supplier Product No.: 009-001-t10s

Supplier: Rockland

Purpose: PTK9 recombinant protein-GST fusion protein

Purification: Recombinant human PTK9 (2-end) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.

Application Details

Application Notes:

Application Note: PTEK9 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. PTK9 Protein is suitable for use in Western Blot. Expect a band approximately ~64 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: PTEK9 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: TWF1 (Twinfilin, Actin-Binding Protein 1 (TWF1))

Alternative Name: TWF1

Background:

Synonyms: Twinfilin 1, TWF1, A6, Twinfilin-1, Protein A6, Protein tyrosine kinase 9, TWF1, PTK9

Background: PTK9 is an actin monomer-binding protein conserved from yeast to mammals which has been localized to cortical G-actin-rich structures that may be regulated by the small GTPase RAC1. PTK9 has tyrosine kinase activity similar to that of recombinant FGFR2, with optimal activity over pH 6.5 to 7.4 and a preference for manganese over magnesium as a divalent cofactor (1). PTK9 plays a central role in regulation of actin dynamics and cell motility in lymphoma cell homeostasis in vivo and represent as a bona fide lymphoma drug target (2). PTK9 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cancer, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, and Neurobiology research.

NCBI Accession: NM_002822

Pathways: Regulation of Actin Filament Polymerization, Maintenance of Protein Location