ARAF Protein (GST tag)

Catalog No. ABIN6700850

Supplier Product No.: 009-001-r41s

This Recombinant ARAF protein is expressed in Insect cells (Sf9).

Quick Overview for ARAF Protein (GST tag) (ABIN6700850)

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >70%

Product Details

Purification tag / Conjugate: This ARAF protein is labelled with GST tag.

Supplier Product No.: 009-001-r41s

Supplier: Rockland

Purpose: ARAF recombinant protein-GST fusion protein

Purification: Recombinant human ARAF (209-end) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.

Application Details

Application Notes:

Application Note: ARAF Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. ARAF Protein is suitable for use in Western Blot. Expect a band approximately ~ 37 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: ARAF Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Alternative Name: ARAF

Background:

Synonyms: PKS2, A-RAF, ARAF1, RAFA1

Background: ARAF belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. ARAF play a critical role in cell growth and development (1).The N-terminal regulatory domain of ARAF interacted with the putative mitochondrial proteins TOM and TIM44. ARAF is expressed predominantly in urogenital tissues (2). The complete coding sequence of the human A-raf-1 oncogene and transforming activity of a human A-raf carrying retrovirus. ARAF Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, and Cancer research.

NCBI Accession: NM_001654

Pathways: MAPK Signaling, RTK Signaling, Hepatitis C