FGF2 Protein

Catalog No. ABIN6700922

Supplier Product No.: 009-f01-u84-0050

This Recombinant FGF2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for FGF2 Protein (ABIN6700922)

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 97,00%

Grade: Animal-Free

Product Details

Supplier Product No.: 009-f01-u84-0050

Supplier: Rockland

Purpose: Human Fibroblast Growth Factor 154 basic Recombinant Protein (Animal Free)

Purification: Fibroblast Growth Factor is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-PAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 1 ng/mL.

Application Details

Application Notes:

Application Note: Fibroblast Growth Factor 154 basic Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Fibroblast Growth Factor 154 basic in immunological assays. Lyophilized from 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 50μL

Buffer: Lyophilized from 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Alternative Name: FGF2

Background:

Synonyms: Heparin-binding growth factor 2 (HBGF-2), Prostatropin, Basic fibroblast growth factor (bFGF)

Background: Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Although the mechanism remains unclear, FGF-basic 154, also known as FGF-2, is a critical component in keeping embryonic stem cells undifferentiated in cell culture systems. Recombinant human FGF-b 154 (FGF-2) is a non-glycosylated protein, containing 154 amino acids, with a molecular weight of 17.2 kDa.

UniProt: P09038

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins