IL-17A/F Protein

Catalog No. ABIN6700928

Supplier Product No.: 009-001-v56-0005

This Recombinant IL-17A/F protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-17A/F Protein (ABIN6700928)

Target: IL-17A/F (Interleukin-17 A/F Heterodimer (IL-17A/F))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Product Details

Supplier Product No.: 009-001-v56-0005

Supplier: Rockland

Purpose: Human Interleukin-17AF Heterodimer Recombinant Protein

Purification: Interleukin-17AF Heterodimer purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by a dose-dependent production of IL-6 in cultured mouse NIH 3T3 fibroblasts and is typically 3-15 ng/mL.

Application Details

Application Notes:

Application Note: Interleukin-17AF Heterodimer Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-17AF Heterodimer in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL-17A/F (Interleukin-17 A/F Heterodimer (IL-17A/F))

Alternative Name: IL17A/IL17F

Background:

Synonyms: IL17 heterodimer, IL17AF heterodimer, CTLA-8 ML-1 dimer, Interleukin 17AF, Interleukin-17AF heterodimer

Background: Interleukin-17AF (IL-17AF) is a member of the IL-17 family of proteins produced by a subset of T cells, called Th17, following stimulation with IL-23. Since IL-17AF is thought to signal through the IL-17RA receptor, its biological function is similar to that of IL-17A in that it induces the production of a variety of chemokines, in addition to airway neutrophilia. In regard to these functions, IL-17AF has less activity than the IL-17A homodimer but, greater activity than the IL-17F homodimer. Human and rat IL-17AF both show activity on mouse cells. Recombinant human IL-17AF is a non-glycosylated heterodimer, containing one IL-17A subunit and one IL-17F subunit. The dimer has a total of 271 amino acids, with an approximate molecular weight of 30.7 kDa.

UniProt: Q16552