IL-17A/F Protein (AA 24-155) (His tag,AVI tag,Biotin)

Catalog No. ABIN6253197

Product Details

Target: IL-17A/F (Interleukin-17 A/F Heterodimer (IL-17A/F))

Protein Type: Recombinant

Protein Characteristics: AA 24-155

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL-17A/F protein is labelled with His tag,AVI tag,Biotin.

Sequence: AA 24-155

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: IL-17A/F (Interleukin-17 A/F Heterodimer (IL-17A/F))

Alternative Name: IL-17A & IL-17F (IL-17A/F Products)

Synonyms: il17-5 Protein, si:ch211-63o20.2 Protein, interleukin 17a/f3 Protein, il17a/f3 Protein

Background: Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Molecular Weight: 34.1 kDa